Abstract
The objective was to elucidate the effects of dietary guanidinoacetic acid (GAA) supplementation on broiler performance, serum enzymes, oxidative biomarkers, mitochondrial activities, carcase traits, gross lesion of cardiac muscle and liver histopathology in broilers challenged with T-3-hormone. A total-of-192 one-day-old mixed sexed broilers were randomly assigned in a two factorial design, including two dietary treatments; control diet supplemented with or without T-3-hormone (1.5 ppm) and GAA diet (0.06%) supplemented with or without T-3-hormone (1.5 ppm). Each group was subdivided into eight replicates. Results showed interactions between GAAxT(3)-hormone. GAA diet significantly mitigated the negative effect of T-3-hormone on serum total creatine kinase (CK), cardiac muscle (CK-MB), liver malondialdehyde (MDA) and superoxide dismutase (SOD), mitochondrial activities of cardiac muscle and liver histopathological lesion. In conclusion, GAA at a rate of 0.06% may have the potential to mitigate the negative effect of dietary T-3-hormone but could not reduce the ascites mortality at such inclusion rate.