Abstract
The effects of limonene exposure on the growth of Microcystis aeruginosa and the release of toxic intracellular microcystin (MCY) were tested by evaluating the results obtained from the batch culture experiments with M. aeruginosa FACHB-905. The time series of cell as well as intracellular and extracellular MCY concentrations were evaluated during 5 d of the incubation. After exposure to limonene, the number of cells gradually diminished; the net log cell reduction after 5 d of the exposure was 3.0, 3.6, and 3.8 log when the initial cell densities were set at 1.6 x 10(7), 1.1 x 10(6) and 4.1 x 10(5) cell/mL, respectively. Limonene was found to significantly influence the production and release of MCY. As the limonene exposure could inhibit the increase in the number of cells, the increase in the total MCY concentration in the medium was also inhibited. In the presence of limonene, the intracellular MCY was gradually released into the medium through a gradual reduction in the number of cells. The extracellular MCY concentration in the medium was significantly higher in the limonene-exposed samples than in the control samples, which confirmed that limonene cannot decompose the extracellular MCY. (c) 2014 Elsevier Inc. All rights reserved.