Abstract
Magnetite Fe3O4 and spinel (2:1) and (4:1) NiFe2O4 magnetic nanoparticles (MNPs) were prepared by simple and affordable co-precipitation methods using an extract of star anise (Illicium verum) as a green reducing agent. The morphology and chemical composition of these MNPs were confirmed by field-emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, UV-visible spectroscopy, and X-ray diffraction (XRD). The synthesized magnetite Fe3O4 and spinel (2:1) and (4:1) NiFe2O4 MNPs were in the size range of 0.1-1 mu m. The MNPs had irregular clustered platelets (magnetite Fe3O4) and pyramidal structures (spinel (2:1) and (4:1) NiFe2O4 NPs). The average sizes of the synthesized magnetite Fe3O4, and spinel (2:1) and (4:1) NiFe2O4 MNPs calculated using XRD analysis were 66.8, 72.5, and 72.9 nm, respectively. In addition to the characteristic absorption peaks of magnetite Fe3O4, those of spinel (2:1) and (4:1) NiFe2O4 MNPs were detected at similar to 300-350 nm and similar to 700 nm, respectively. Overall, the results of this study indicate that the synthesized magnetite Fe3O4, and spinel (2:1) and (4:1) NiFe2O4 MNPs showed high biomedical activities against liver carcinoma cells and non-small lung adenocarcinoma cells.