Abstract
The reproductive efficiency of camels can be improved using artificial insemination and embryo transfer programs that require a dependable method for synchronization of follicular waves. This study aimed to evaluate the efficacy of a progesterone-releasing intravaginal device (PRIDΔ®) to induce synchronization for the follicular wave in Camelus dromedarius during the rutting season, by ovarian monitoring, assessing sexual receptivity and fertility, and quantifying progesterone (P4) and estradiol (E2) concentrations. Twenty animals received a new PRIDΔ with 1.55 g of P4 for 2 wk (Day 14); another 20 camels were used as the control. Ultrasound ovarian monitoring and camel sexual receptivity were evaluated each day during the course of the experiment for all camels in the two groups. The proportion of animals in the ovulatory (follicle diameter: 12 to 18 mm) and non-ovulatory phases was calculated according to the ovarian monitoring results using ultrasound. Blind natural mating occurred on Day 16, and ovulation, non-return, and fertility rates were calculated. Blood samples were collected. Serum P4 and E2 levels were measured daily during the trial period (21 d) using ELISA kits. The results revealed that the proportion of females in the ovulatory phase on Day 16 in the PRIDΔ group was significantly higher than that in the control group (85 and 50, respectively). Serum P4 levels increased significantly after PRIDΔ insertion and reached the maximum values (5.47, 5.02, 5.55, and 4.88 ng/mL) on Days 4, 5, 6, and 7, respectively. P4 levels significantly decreased after PRIDΔ removal and reached the minimal levels (1.23 and 0.67 ng/mL) on Days 15 and 16, respectively. There was no significant difference in the E2 levels between the groups. Ovulation, non-return, and fertility rates in inseminated camels were significantly higher in the PRIDΔ group (85%, 80%, and 75%, respectively) than in the control group (50%, 45%. and 45%, respectively). In the control group, the P4 level was maintained at a minimal level (≥1 ng/mL). In conclusion, the treatment of dromedary camels with PRIDΔ led to a uniform increase in serum concentrations of P4; however, it could not stop follicular growth. After PRIDΔ removal, P4 concentrations dropped and stimulated the development of a new follicular wave; most female camels were in the ovulatory phase 2 d after PRIDΔ removal. Therefore, PRIDΔ is efficient at inducing follicular wave synchronization in C. dromedarius with good fertility.
•The current study is the first study trying to use PRIDΔ (not old PRID) to synchronize follicular wave in camel.•PRIDΔ produced a uniform increase in serum concentration of progesterone.•PRIDΔ could not suppress follicular wave during the treatment.•Most of camels were in breeding phase two days after PRIDΔ withdrawal.•PRIDΔ is efficient to synchronize follicular wave with good pregnancy rate in camel during breeding season.