Abstract
Introduction: Erythrocyte aggregation and blood coagulation are of great interest and are still under investigation by many researchers. Erythrocytes have a direct effect on hemorheological properties. Real-time in vitro studies on blood coagulation and aggregation provide a chance to understand their mechanisms in normal and pathological conditions. Additionally, this method offers control over the physical and chemical conditions during the study.
Objective: The present study introduced a simple in vitro technique to study blood aggregation and coagulation under controlled conditions.
Methods: The technique used in this study is based on the measurement of the electrical properties of blood. A simple flow chamber was made from two cylinders with a gap between them. The outer cylinder remains stationary, and the inner cylinder rotates about its axis. The inner cylinder velocity is controlled by a stepper motor. Blood samples are introduced in the gap between the two cylinders. Capacitance and impedance of blood samples were recorded by two electrodes attached to the outer cylinders and in direct contact with blood.
Results: Quantitative parameters were extracted from the capacitance and impedance time courses. These parameters were used to describe the aggregation and coagulation processes under different shear rates. Strong correlations between the aggregation index and shear rate were found for normal and diabetic blood samples. Additionally, strong negative correlations of coagulation time were found for normal and diabetic blood samples. In conclusion, the electrical analysis of blood reflects well the interactions between internal blood contents.
Conclusion: The parameters extracted from this technique can be used in the quantitative description of hemorheological processes under different physical conditions.