Abstract
An ultrasenstive electrochemical probe was suggested for the estimation and quantification of gemifloxacin mesylate (GFX) in its pharmaceutical products and biological media. Differential pulse (DPP), Cyclic voltammtery (CV) and Alternative current ACt were exploited to investigate the electrochemical nature of GFX. Over the pH range of 2.6-10, the investigated drug demonstrated significant cathodic peaks. The best polarographic response was achieved in acetate buffer (pH 5), scan rate 15 mV s-1 and pulse amplitude -90 mV. The outcome linearity was 1.59×10-6 - 2.70×10-5 mol L-1 (0.77 – 13.1 μg mL-1). The limits of detection and quantification were determined as 2.89×10-7 and 8.76×10-7 mol L-1, respectively. The proposed electrochemical approach was validated and successfully used to quantify the selected drug in different media. The outcome results were compared with others obtained from reference method and displayed good accuracy and precision agreement.