Abstract
The electrochemical impedance spectroscopy, EIS, was used to investigate the interfacial behavior of bovine serum albumin (BSA) on different titanium (Ti) surfaces at open circuit potential. Nano porous titanium oxide film, TiO2, was anodically formed on titanium from electrolyte containing 1M H2SO4 and 0.5% wt NaF at 20 V for 20 min. The biomimetic calcium-phosphate coating was deposited cathodically on anodized titanium, (AT) from supersaturated calcium-phosphate solution, SCPS, at low temperature, 60 degrees C. To be the Ti, AT, and Ca-P/AT surfaces have been used to compare among microstructures and protein adsorption behavior. Island from calcium-phosphate coating was detected by the scanning electron microscope, SEM. The electron diffraction X-ray analysis, EDX, was used to investigate the difference in chemical structures between different types of examined surfaces. The experimental impedance data were fitted to theoretical data according to proposed equivalent circuit models. The impedance data fitting enabled to explain the adsorption phenomena of BSA occurred on the different specimen's surface. This work indicated formation of nano-TiO2 film on Ti improved the Ca-P induction ability which enhanced the adsorption of BSA on its surface.