Abstract
The genus enterovirus is constituted by a wide number of viral species having in common many of morphological and genetic characters. These viruses present a high epidemiological power and are in question of various human diseases. The absence of easy and standardized technique for their detection justify the need to develop new techniques of diagnosis. In this work, we describe the development of an immunosensor based on functionalized gold electrode allowing enteroviruses detection. The biosensor is based on the grafting of specific monoclonal enterovirus antibodies onto functionalized gold electrode. Each step of biosensor construction was characterised by electrochemical impedance spectroscopy, fitted with an equivalent electric circuit and the antibody-virus interaction was measured with a detection limit of 10 viral particles (vp) or 1.4 vp/mL.