Abstract
Carboxylic acid ligand and its triorganotin complexes; investigated for their binding with DNA and bioactivities. Among all tri-n-butyltin complex showed comparatively good DNA binding via intercalation and better antitumor potential.
•Synthesis characterization of carboxylic acid ligand and its triorganotin complexes.•All compounds interact with DNA via intercalation.•Among all comparatively good DNA binding was investigated for APA-3 complex.•APA-3 complex showed better antitumor potential.
A carboxylic acid ligand, (Z)-4-(4-acetylphenylamino)-4-oxobut-2-enoic acid (APA-1), and its triphenyl-(APA-2) and tributyl-tin(IV) (APA-3) compounds have been synthesized and investigated for their binding with ds.DNA using UV–visible spectroscopy, fluorescence spectroscopy, cyclic voltammetry, and viscosity measurements under physiological conditions of pH and temperature. The experimental results from all techniques i.e. binding constant (Kb), binding site size (n) and free energy change (ΔG) were in good agreement and inferred spontaneous compound–DNA complexes formation via intercalation. Among all the compounds APA-3 showed comparatively greater binding at pH 4.7 as evident from its greater Kb values {APA-3: Kb: 5.63×104M−1 (UV); 7.94×104M−1 (fluorescence); 9.91×104M−1 (CV)}. Electrochemical processes of compounds before and after the addition of DNA were found diffusion controlled. Among all compounds, APA-3 exhibited best antitumor activity.