Abstract
The world has heavily relied on fossil fuels for decades to supply energy demands. However, the usage of fossil fuels has been strongly correlated with impactful problems, which lead to global warming. Moreover, the excessive use of fossil fuels has led to their rapid depletion. Hence, exploring other renewable and sustainable alternatives to fossil fuels is imperative. One of the most sustainable fossil fuel alternatives is biofuel. Microalgae-based biofuels are receiving the attention of researchers due to their numerous advantages compared with those obtained from other types of feedstocks. Hence, it is essential to explore the recent technologies for biofuel produced from microalgae species and define the possible challenges that might be faced during this process. Therefore, this work presents the recent advancements in biofuel production from microalgae, focusing on emerging technologies such as those using nanomaterials and genetic engineering. This review focuses on the impact of nanoparticles on the harvesting efficiency of various microalgae species and the influence of nanoparticles on biofuel production. The genetic screening performed by genome-scale mutant libraries and their high-throughput screening may assist in developing effective strategies for enhancing microalgal strains and oil production through the modification of enzymes. Furthermore, the barriers that limit the production of biofuels from microalgae are introduced. Even though microalgae-based biofuels are perceived to engage with low negative impacts on the environment, this review paper touches on several environmental issues associated with the cultivation and harvesting of microalgae species. Moreover, the economic and technical feasibility limits the production of microalgae-based biofuels.