Abstract
This study introduced a simple method developed for enantioselective extraction of D-phenylalanine prior to its determination by high performance liquid chromatography. The method was based on silica gel developed by surface immobilized new chiral ionic liquid (SG-L-PhAlaC(2)NTf(2)). Surface characteristics of the synthesized SG-L-PhAlaC(2)NTf(2) phase were confirmed by both FT-IR spectroscopy and scanning electron microscope. Results of the selectivity study and effect of pH on SG-L-PhAlaC(2)NTf(2) phase showed that SG-L-PhAlaC(2)NTf(2) was the most enantioselective toward D-phenylalanine. Data of adsorption isotherm study revealed that the adsorption capacity of SG-L-PhAlaC(2)NTf(2) for D-phenylalanine was the most by 97.35% as compared to activated silica gel at pH value of 3.0 and after one hour contact time. Results demonstrated that the adsorption of D-phenylalanine onto SG-L-PhAlaC(2)NTf(2) phase well followed with Langmuir adsorption isotherm and pseudo second-order kinetic models. The efficiency of the methodology was ultimately validated by implementing it to real water samples with satisfactory results.