Abstract
Micro propagation has the potential to provide very high multiplication rates of selected true genotypes. It allows rapid production of high quality, disease free and uniform planting materials irrespective of weather or season. The paper highlights studies on the same, outlines potential of using single to multiple co-cultures combinations of microbe that were in symbiotic relationship with plants and thus promoting greater nutritional assimilation by plants. The microorganisms were obtained from six different soil locations and were identified using gram staining and IMVIC techniques. Later they were enumerated using the serial dilutions. These bacteria were then inoculated to the tissue cultured plants in a series of triple combination and in different concentrations. The result obtained was compared against the control. The key parameters of plant growth are reported and discussed. There is enough experimental evidence with bacteria (bacterization) and vesicular arbuscularmycorrhiza (mycorrhization) inoculations to recommend utilization of this technology in commercial micropropagation. There was an increased growth in all the macro and micro nutrients and protein formation and increased the immunity and performance of the cultured plants, fidelity test was conducted to prove that plants had the same genetic make up. Techniques have found increasing use in the conservation of agricultural crops and threatened plants in recent years and this trend is likely to continue as it is significantly contributing to agricultural growth.