Abstract
Coal gasification wastewater (CGW) contains high concentration phenols which lead to poor anaerobic biodegradability and resource utilization. In this paper, new insights to improve synthetic CGW anaerobic degradation with the help of graphene under co-digestion conditions were investigated. Batch tests showed that with the addition of graphene dosage of 10 g/L and glucose as a co-substrate with chemical oxygen demand (COD) concentration of 2000 mg/L, the average COD concentration decreased from 3995 mg/L on day 1 to 983 mg/L on day 12. The average total phenol (TP) concentration decreased from 431 mg/L on day 1 to 23 mg/L on day 12. The cumulative methane production for 12 days was about 200 mL. Long-term experiments showed the average effluent COD and total phenol reached 1137 mg/L and 200 mg/L, respectively. While methane production stabilized at 500 mL/d. In addition, the coenzyme F
concentration increased from 1.075 μmol/g/VSS to 2.3 μmol/g/VSS. The analysis of microbial community structure indicated that the performance of phenols removal and methane production was related to the main microbial flora. The enriched Clostridium, Pseudomonas and species from Firmicutes and Chloroflexi participated in the stages of hydrolysis and acidogenesis. The electrogens Pseudomonas and archaea Methanosaeta were likely the major groups taking part in the direct interspecies electron transfer (DIET). The results obtained in this paper provide a theoretical basis for high-efficiency anaerobic degradation of CGW in practical engineering applications.