Abstract
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•Improved endoglucanse production via SSF using paddy straw in presence of nanocomposite.•Enhanced functional stability of endoglucanse via treatment with nanocomposite.•Mn-oxide based porous nanocomposite is synthesized using mixed fruit waste employed as reducing agent.•Work has significant waste valorization for bioconversion and nanocatalyst preparation.•The study supported the contribution of bioresources towards carbon neutrality.
Microbial cellulases are the enzymes used in numerous industrial biotechnological applications. Efficiency of celluloytic cocktails plays a key role in the conversion of biomass into biofuels, but limited production, high cost and low efficiency are the main obstacles to sustainable biorefining. The current work aims to establish a feasible approach for boosting the production of fungal endoglucanse (EG) and its functional stability utilizing nanocomposite materials based on manganese oxide. Herein, aqueous extract from mixed fruit waste was used to synthesize the nanocomposite sample, which was subsequently subjected to several characterization techniques for analysis. Following the solid-state fermentation of paddy straw, and by employing 75 mg nanocomposite, 192 IU/gds EG was produced under the optimal conditions, while 19 IU/gds FP and 98 IU/gds BGL production were recorded. The crude EG enzyme treated with nanocomposite also shows complete stability at pH 5.0 for 3.5 h while retaining thermal activity at 70 °C for 4 h.