Abstract
ØAs a natural source, Sargassum longifolium seaweed was used.ØCuO-NPs of 40 to 60 nm have been reported.ØFor antibacterial screening, the CuO NPs showed substantial activity.ØDPPH testing was adopted for free radical scavenging.
In this investigation, interaction between the extract of brown algae Sargassum longifolium (S. longifolium) and copper sulphate (CuSO4) was examined. The CuSO4 (Cu2+) has been reduced to Cu in the presence of secondary metabolites present in algae and it reacted with atmospheric oxygen to provide copper oxide nanoparticles (CuO-NPs). The chemical composition was quite well determined by several studies. The preliminary analysis was the colour change of the solution. The SPR (Surface Plasmon Resonance) band was discerned with the help of UV – Visible absorption spectrophotometer at a wavelength of 350 nm. SEM (Scanning Electron Microscopy) and TEM (Transmission Electron Microscopy) allow the visualization of the distribution of CuO-NPs. The prepared nanoparticles size was perceived to be 40 – 60 nm. XRD (X-ray diffraction) substantiated the crystallinity of the synthesized CuO NPs. The pathogenicity of CuO-NPs was studied against Vibrio parahemolyticus, Aeromonas hydrophila, Serratia marcescens and Vibrio harvey through agar well diffusion method. The CuO-NPs also exhibits good antioxidant properties. Interesting results showed the possibility of one alternative to biological activities by the green innovative synthesis and their combination with an essential antimicrobial.