Abstract
An
32.2 invasive group A streptococcus (iGAS) outbreak occurred in Liverpool from January 2010 to September 2012. This genotype had not previously been identified in Liverpool, but was responsible for 32% (14/44) of all iGAS cases reported during this time period. We performed a case-case comparison of
32.2 iGAS cases with non-
32.2 control iGAS cases identified in the Liverpool population over the same time period to assess patient risk factors for
32.2 iGAS infection. The
32.2 iGAS cases were confined to the adult population. We show that homelessness, intravenous drug use, and alcohol abuse predisposed patients to
32.2 iGAS disease; however, no obvious epidemiological linkage between the patients with
32.2 iGAS could be identified. Comparative whole-genome sequencing analysis of
32.2 iGAS and non-
32.2 control isolates was also performed to identify pathogen factors which might have driven the outbreak. We identified 19 genes, five of which had previously been implicated in virulence, which were present in all of the
32.2 iGAS isolates but not present in any of the non-
32.2 control isolates. We report that a novel
32.2 genotype emerged in Liverpool in 2010 and identified a specific subset of genes, which could have allowed this novel
32.2 genotype to persist in a disadvantaged population in the region over a 3-year period.