Abstract
In the present study, Fenugreek plants obtained from two different cultivars, one from Saudi Arabia and another from Yemen, were screened for phytochemical active constituents and investigated for antimicrobial properties against a selection of grampositive and gram-negative pathogenic bacteria. Five different solvent seed extracts from each cultivar were tested. The results of the study demonstrated that the chloroform and methanolic extracts possessed significant antibacterial activity against Escherichia coli ATCC25922, Pseudomonas aeruginosa ATCC27853, Staphylococcus aureus ATCC25923, Salmonella typhi ATCC14027 and Klebsiella pneumonia ATCC700603. The antimicrobial activity of the extracts was investigated using the agar well diffusion method. The results of the antimicrobial analysis identified Shigella sonnei as the most sensitive pathogen to the crude extracts of Fenugreek seeds obtained from Saudi Arabia and with the largest zones of inhibition.