Abstract
The current study was performed with aim of evaluating antioxidant, cytotoxicity, a-amylase, and alpha-glucosidase inhibitory activities and mutagenicity properties of Martynia annua mediated Chitosan nanoparticles (MAL-CNPs). The green synthesized MAL-CNPs were characterized and confirmed through several characterization techniques, including UV-visible spectroscopy (UV-Vis), high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and dynamic light scattering (DLS). The HR-TEM analysis exhibited that the as-synthesized chitosan nanoparticles are spherical in shape. Furthermore, the DLS analysis exhibited that the average size of MAL-CNPs was 53 nm and the maximum diameter was 130.7 nm. The antioxidant activity results revealed that the MAL-CNPs showed DPPH (2,2-diphenyl-1-picrylhydrazyl) (66.78%) and H2O2 (91.65%) scavenging activities at 50 mu g/mL concentration. The IC50 values were 2.431 mu g/mL and 50 mu g/mL for DPPH and H2O2, respectively. MTT (3-4, 5 dimethylthiazol-2yl-2, 5-diphenyltetrazolium bromide) assay results exhibited dose-dependent cytotoxicity found from 50 mu g/mL concentration of MAL-CNPs. The MAL-CNPs showed remarkable alpha-glucosidase and alpha-amylase inhibitory activity (IC50 1.981 mu g/mL and 161.8 mu g/mL). No toxic effect of MAL-CNPs was found through the Ames test. Further, the study concluded that MAL-CNPs are non-toxic and possess adequate antioxidants and cytotoxicity activity against cancer cells, alpha-glucosidase, and alpha-amylase inhibitory activity. Hence, the MAL-CNPs were considered for biomedical applications after the assessment of their efficiency and safety.