Abstract
A simultaneous separation process of two anticancer drugs carboplatin and temozolamide in their binary mixture using capillary zone electrophoresis method has been introduced in this study. Under full optimized conditions a fused silica capillary was used to carry out the electrophoretic separation using a background electrolyte 50 mmol L-1 acetate buffer of pH = 3.6 which was run at potential 20 kV, hydrodynamic injection 8 s and 60 mbar pressure. The capillary cartridge temperature was adjusted at 30 degrees C and a diode array detector was utilized to record the separation peaks in the presence of internal standard phenylethylamine solution at 330 nm. Excellent electrophoretic separation was achieved over linear concentration ranges of 10-240 and 5-100 mu g mL(-1) with correlation coefficients of 0.9996 and 0.9993 for carboplatin and temozolamide, respectively. The obtained detection and quantification limits were found to be 2.5 and 7.4 mu g mL(-1) for carboplatin, while, for temozolamide were found to be 1.3 and 4.3 mu g mL(-1). This analytical approach was validated and the results were compared with those reported by other reported methods.