Abstract
Background: Nano-biotechnology is considered as one of the main vigorous fields of research in new material science. Recently, biosynthetic methods employing both biological microorganisms such as bacteria and fungus or plant extracts have developed rapidly as a trouble-free and feasible choice to obtain nanomaterials alternative to more complex chemical synthetic procedures. The particular distinctiveness like size, allocation and shape give the nanoparticles different properties from bulk material.
Objectives: Eruca sativa and Spinacia oleracea plants were evaluated for their extra cellular potential synthesis of silver nanoparticles and their bactericidal impact on Streptococcus pneumoniae and Pseudomonas aeruginosa.
Materials and Methods: Aqueous solutions of AgNO3 were mixed with plant extracts. Transmission electron microscopy (TEM) was used to characterize the morphology of the nanoparticles obtained from plant extracts. Energy dispersive X-ray (EDX) spectrometer established the existence of elemental sign of the silver and homogenous allocation of silver nanoparticles. Diffraction by using X ray (XRD) analysis for the formed AgNPs revealed spherical plus cubical structures with different planes ranging between 111 to 311 planes. Scanning electron microscopy (SEM) was used to characterize the morphology of the nanoparticles obtained from plant extracts.
Results: The antibacterial action of AgNPs against human pathogens, Streptococcus pneumoniae and Pseudomonas aeruginosa was recognized. Our work showed a rapid, eco-safe and suitable method for the synthesis of AgNPs from Eruca sativa and Spinacia oleracea leaf extracts and can be used for pharmaceutical and other biomedical applications.
Conclusions: It can be suggested that AgNPs show effective antibacterial properties owing to their exceptionally big exterior region, which provides superior contact with microorganisms and its interactions with bacteria are and localized on the membrane of the organism.