Abstract
Paclitaxel (PTx) and D-L Sulforaphane (SFN) natural derivatives could inhibit many types of cancerous cells proliferation through apoptosis and autophagy. Toxicity of PTx and SFN to Caco-2 cells was concentration dependent using MTT assay. Sole SFN was significantly toxic than sole PTx. PTx and SFN combination induced a synergetic potential of PTX toxicity than sole form and the recorded IC50 in mu gm / ml was in the order of (862.8,2.,8 and 22.37) for sole PTx, SFN and combined form respectively. PTx and SFN sole and combined form induced Caco-2 cells DNA accumulation at the G2/M phase but not significantly at the Go/G1 and S phases. Apoptosis was proved via a significant up regulation of P53, Cy-c, Bax, casp-3 and down regulation of Bcl-2 with elevated Bax/Bcl-2 ratio. Antioxidant activity supported apoptotic potential showed significantly elevated ROS, MDA and reduced GSH.