Abstract
The purpose of this study was to find the most cadmium (Cd2+) tolerant and remediated bacteria isolate from KNO3 processing unit contaminated soil. One isolate out of 19 isolates possessed excellent Cd2+ tolerance than others, which was recognized as Enterobacter hormaechei SFC3 through molecular characterization (16S rRNA sequencing). The identified E. hormaechei SFC3 contained 55% and 45% of GC and AT content, respectively. The wild and acridine orange mutated E. hormaechei SFC3 exhibited excellent resistance to Cd2+ up to the concentration of 1500 μg mL−1. Furthermore, the wild E. hormaechei SFC3 and mutated E. hormaechei SFC3 showed 82.47% and 90.21% of Cd2+ remediation on 6th days of treatment respectively. Similarly, the Cd2+ tolerant wild and mutated E. hormaechei SFC3 showed considerable resistance to all the tested antibiotics. The findings indicate that E. hormaechei SFC3 isolated from KNO₃ processing unit contaminated soil is a promising candidate for microbial remediation of Cd2+ contamination.
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•First report on enumerating Cd tolerant bacteria from firework industry polluted soil.•Enterobacter hormaechei SFC3 was found as predominant Cd tolerant bacteria.•Acridine orange (500 μg mL−1) mutated E. hormaechei SFC3 showed excellent tolerance.•Wild & acridine orange mutated E. hormaechei SFC3 showed 82 & 90% of Cd2+ remediation.•Both strain showed considerable resistance to various tested antibiotics.