Abstract
The various physio- and biochemical parameters including photosynthetic rate, stomatal conductance, transpiration rate, relative average growth, chlorophyll content, soluble sugar and proline content were investigated on mutant Hordeum vulgare obtained from first generation upon two doses of gamma-rays, viz., 300 and 350 Gy, respectively. The level of stress marker "proline" was accumulated higher whereas physiological parameters were decreased at these doses as compared to control ones. Thus, gamma-rays irradiations have strong mutagenic impact which led to generation of reactive oxygen species (ROX) on barley and its mutagenecity could be interesting to understand this mechanism of mutation.