Abstract
Herein, an efficient analytical method based on an extraction protocol was developed and validated for the spectrophotometric detection of phosphate (PO43−) in chilled and frozen chicken samples. The preparation was a very simple sample that included freeze-drying, extraction with acidified amino acids and filtration of the final extract prior to spectrophotometric analysis. FT-IR spectrum of CS1 was confirmed that the selected solvent has successfully extracted PO43− from the chilled chicken sample. Analytical performances were assessed in chilled of one of the merchant species and showed good recoveries with relative standard deviations (RSD) below 2%. The analytical validation parameters of the method in terms of limit of quantification (LOQ) and detection (LOD) were calculated and found to be 0.10 and 0.032 mg/L, respectively. This protocol has been effectively applied to PO43− determination in chicken samples collected from Saudi supermarkets (Northern KSA), and PO43− in all frozen samples was detected at high mg/kg levels ranging from 2.11 × 104 to 2.90 × 104 mg/kg, while in limit levels of PO43− concentration were detected in the chilled samples except chilled sample 1 (CS1), which was 2.22 × 104 mg/kg. The extraction and determination protocol suggested that this developed method could be validated for routine monitoring of PO43− in food quality control laboratories and safety monitoring.