Abstract
•This is the first study to assess the efficiency of LUT7G against diverse gastric ulcer models.•LUT7G shows significant gastroprotective activity.•LUT7G pretreatment preserves normal gastric functions on ulcerated rats.•Possible mechanisms were anti-inflammatory, antioxidant and anti-apoptotic actions.
The gastroprotective effect of luteolin-7-O-glucoside (LUT7G) on different ulcer models using rats including the indomethacin model, the ethanol-induced gastric ulcer model and the Shay ulcer model was examined. The ethanol-induced ulcer group showed significant increases in MPO, NO, iNOS, MMP-2, MMP-9, caspase-3, apoptosis, IL-6, TNF-α, IKK, NF-κB p65, and ICAM-1 and declines in PGE2, arginase, SOD, GSH, mucin, IL-10, eNOS, COX-1, HSP-70, and IκBα levels. However, LUT7G (25 mg/kg) pretreatment significantly reverted the pathophysiological levels of these biomarkers to near normal levels. The gastroprotective activity of LUT7G was abolished by pretreatment with SC560, rofecoxib, and L-NAME, demonstrating the participation of COX and NOS in LUT7G-facilitated gastroprotection against ethanol-induced ulcers. Convincingly, LUT7G (25 mg/kg) provided protective effects in the rat gastric mucosa against ethanol-induced gastric injury at least in part to antisecretory, anti-inflammatory, antioxidative, and antiapoptotic activity, and augmentation of PGE2, mucin and HSP-70 synthesis.