Exploitation of Mangrove Endophytic Fungi for Infectious Disease Drug Discovery

Danielle H Demers; Matthew A Knestrick; Renee Fleeman; Rahmy Tawfik; Ala Azhari; Ashley Souza; Brian Vesely; Mandy Netherton; Rashmi Gupta; Beatrice L Colon; Christopher A Rice; Mario A Rodríguez-Pérez; Kyle H Rohde; Dennis E Kyle; Lindsey N Shaw; Bill J Baker

doi:10.3390/md16100376

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Exploitation of Mangrove Endophytic Fungi for Infectious Disease Drug Discovery

Journal article

Open access

Peer reviewed

Exploitation of Mangrove Endophytic Fungi for Infectious Disease Drug Discovery

Danielle H Demers, Matthew A Knestrick, Renee Fleeman, Rahmy Tawfik, Ala Azhari, Ashley Souza, Brian Vesely, Mandy Netherton, Rashmi Gupta, Beatrice L Colon, …

Marine drugs, Vol.16(10), p.376

10/10/2018

DOI: https://doi.org/10.3390/md16100376

PMCID: PMC6212984

PMID: 30308948

Abstract

Animals

Anti-Bacterial Agents - pharmacology

Bacteria - drug effects

Cell Line

Communicable Diseases - drug therapy

Drug Discovery - methods

Endophytes - metabolism

Fungi - metabolism

Histone Deacetylase Inhibitors - pharmacology

Methyltransferases - antagonists & inhibitors

Mice

There is an acute need for new and effective agents to treat infectious diseases. We conducted a screening program to assess the potential of mangrove-derived endophytic fungi as a source of new antibiotics. Fungi cultured in the presence and absence of small molecule epigenetic modulators were screened against and the ESKAPE panel of bacterial pathogens, as well as two eukaryotic infective agents, and . By comparison of bioactivity data among treatments and targets, trends became evident, such as the result that more than 60% of active extracts were revealed to be selective to a single target. Validating the technique of using small molecules to dysregulate secondary metabolite production pathways, nearly half (44%) of those fungi producing active extracts only did so following histone deacetylase inhibitory (HDACi) or DNA methyltransferase inhibitory (DNMTi) treatment.

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Title: Exploitation of Mangrove Endophytic Fungi for Infectious Disease Drug Discovery
Creators - without role: Danielle H Demers - University of South Florida
Matthew A Knestrick - University of South Florida
Renee Fleeman - University of South Florida
Rahmy Tawfik - University of South Florida
Ala Azhari - University of South Florida
Ashley Souza - University of South Florida
Brian Vesely - University of South Florida
Mandy Netherton - University of Central Florida
Rashmi Gupta - University of Central Florida
Beatrice L Colon - University of South Florida
Christopher A Rice - University of South Florida
Mario A Rodríguez-Pérez - Instituto Politécnico Nacional
Kyle H Rohde - University of Central Florida
Dennis E Kyle - Department of Global Health, University of South Florida, Tampa, FL 33613, USA. DENNIS.KYLE@uga.edu
Lindsey N Shaw - University of South Florida
Bill J Baker - University of South Florida
Publication Details: Marine drugs, Vol.16(10), p.376
Grant note: AI103715 / National Institutes of Health AI103673 / National Institutes of Health R21 AI119787 / NIAID NIH HHS
Identifiers: 9938187208331
Academic Unit: King Abdulaziz University
Language: English
Resource Type: Journal article