Abstract
This study was done to study the possibility of producing high quality, universal or regional commercial inoculants. Sequences of 16SrRNA, glnII and recA genes of rhizobia isolated from different sites in Gadarif State, Sudan were aligned using A plasmid Editor (APE) software to choose the most genetically related strains. The result showed that Rhizobium sp. Haw1 and Rhizobium sp. G6-11 are identical according to the 16SrRNA and glnII genes sequences. They are also almost identical in their recA sequences because they were found differ in one position only. At the same time, these two strains were found differ at 9 positions when compared with Rhizobium sp. UoG27 also isolated from groundnut grown in another location. On the other hand, the sequences alignment of Rhizobium sp. Sab 13 (isolated from nodules of Bambara groundnut) and Rhizobium sp. Umk34 (isolated from nodules of cowpea) also isolated from different locations showed that there are one and five positions of differences in the sequences of 16SrRNA and glnII, respectively. The study concluded that the relationship between rhizobia strains isolated from different sites and different related legumes can be exploited to produce universal or regional high quality inoculants