Abstract
The present study aimed to determine the degradation and transformation of three-ring PAHs phenanthrene and anthracene byCryptococcussp. MR22 andHalomonassp. BR04 under halophilic conditions. The growth progress ofCryptococcussp. MR22 andHalomonassp. BR04 on anthracene and phenanthrene was monitored by colony-forming unit (CFU) technique. The growth of the bacteria was maintained at a maximum concentration of 200 mg/L of all tested hydrocarbon, indicating thatCryptococcussp. MR22 andHalomonassp. BR04 significantly perform in the removal of the PAH-contaminated medium at low concentrations. The fit model to represent the biodegradation kinetics of both PAHs was first-order rate equation The extract prepared from cells supplemented with three different substrates exhibited some enzymes such as hydroxylase, dioxygenase, laccase and peroxidase. The results suggest that both strains had an impressive ability in the degradation of aromatic and aliphatic hydrocarbon but also could tolerate in the extreme salinity condition.