Abstract
A new extractive spectrophotometric method for determination of anti-viral drug ribavirin was developed and validated. The proposed method was based on the formation of red colored complex with Eriochrome black-T in acidic medium. The absorbance of extractive organic layer with chloroform was measured at 576 nm. Beer's law was obeyed revealed that the linear concentration range was 2-120 mu g/mL (r = 0.999) with lower detection and quantification limits 0.87 and 1.76 mu g/mL, respectively. The proposed method was validated according to (ICH) guidelines and successfully applied for the determination of the drug in bulk powder and pharmaceutical formulations.