Abstract
777 Oil is a topically applied Ayurvedic formulation used for the effective treatment of psoriasis. The formulation is composed of leaf extract of Wrightia tinctoria and Oleum Cocus nucifera. A selective, sensitive, and reproducible HPLC method was developed for analyzing marker compound of Wrightia tinctoria (Rutin) in 777 Oil for routine standardization purpose. The chromatography was performed on Phenomenex C18 (250x4.6mm, 5.0 mu m particle) column using methanol-water (60:40, v/v) as mobile phase; adjusted to pH 3.0 by orthophosphoric acid. The flow rate was 1.0mL/min. with detection at 360nm. The values of retention times and capacity factor were 3.88 and 0.40, respectively. The calibration plot showed a good linear relationship between response curve and concentration in the range of 1.0-1000.0 mu gmL-1 with regression coefficient 0.9998. The detection (LOD) and quantification (LOQ) limits were found 27.0 and 95.0 ngmL-1 respectively. The statistical analysis proved that the method was precise, reproducible, selective, and accurate for the analysis of rutin in 777 Oil. The developed HPLC method is useful for the qualitative and quantitative estimation of rutin in 777 Oil and other products of traditional systems of medicine.