Abstract
Chloramphenicol is a classical antibiotic, used for the treatment of typhoid fever all over the world. Some potential manufacturers and users dealing with chloramphenicol are contaminating our natural water resources by discharging their effluents. Therefore, a sensitive, inexpensive, fast, and reproducible HPLC-SPE method was developed for the analysis of chloramphenicol in the wastewater. The column used was monolithic, chromolith performance RP-18e, 100-4.6 (100 × 4.6 mm). The mobile phase used was phosphate buffer (100 mM, pH 3.0)-acetonitrile (75:25, v/v) at 1.5 mL/min with UV detection at 275 nm. The retention, separation, and resolution factors of chloramphenicol were 3.13, 2.0, and 4.30, respectively. The percentage recovery of chloramphenicol from wastewater was 94.0%. Frusemide was used as the internal standard to access the percentage extraction of chloramphenicol from wastewater.