Abstract
Dispersed cells from rat submandibular salivary glands were incubated in medium supplemented with different fatty acids, such as stearic (18:0), oleic (
cis 18:1 ω9) and elaidic acid (
trans 18:1 ω9). The exogenous fatty acids were incorporated into the membrane lipids. A comparison of the adenylate cyclase activity in membranes of cells incubated with different fatty acids revealed the following order (from highest to lowest specific activity): stearic, elaidic, oleic. The findings suggest that changes in adenylate cyclase activity in these dispersed membranes enriched with different fatty acids may be related to the possible changes in membrane fluidity. The synthesis of glycoproteins was studied by measuring the incorporation of [1-
14C]-glucosamine into TCA-PTA-precipitable material in the presence or absence of fatty acids. No effect of the exogenous fatty acids on glycoprotein synthesis was observed.