Abstract
We explored the possibility of the cryo-storage of cord blood hematopoietic stem cells (CBHPSC) with respect to the quantity, quality and biologic efficacy of high altitude (HA) region Abha against sea level (SL) region. The results of the post-processed total nucleated cell count was 8.03 +/- 0.31 x 10(7) and 8.44 +/- 0.23 x 10(7) cells in the HA and SL regions respectively. The mean post processing viability of the nucleated cells was about 87.03 +/- 1.39 (HA) and 88.33 +/- 1.55% (SL) while post thaw cells were 85.61 +/- 1.44 (HA) and 86.58 +/- 1.61% (SL) after transient cryo-storage. The proliferation of CBHSCs after thawing were comparable between the HA and SL regions. The results of the colony forming unit (CFU) assays of CFU-E, CFU-GEMM, CFU-GM and BFU-E were comparable between HA and SL in both fresh and post thaw, while a declining trend with viability was significant. The differentiation capability of post thaw samples into adipocytes and osteocytes were comparable between HA and SL regions. Overall from the results, it can be evidenced that HA cord blood collection, processing or storage does not hinder the quality or biological efficacy of the CBHPSC.