Abstract
This method described the determination of tetracycline in blood serum and it is based on the formation of an Eu(III)-tetracycline complex. Another spectrophotometric method2 utilizing the reaction of tetracycline with p-N, N-dimethylphenylenediamine and chloramines T was described. A sensitive liquid chromatographic method (10) has been adopted for the simultaneous determination of tetracycline, oxytetracycline and minocycline in serum. Tetracyclines were separated from other serum components by RPLC with buffered MeOH mobile phase. UV absorbance of the column effluent was monitered at 267 nm.