Abstract
Absorbance and fluorescence spectral pattern of levosulpiride in absence and presence of first row transition metal ions (Mn-Zn) has been studied at room temperature under physiological condition. The fluorescence spectra of the drug in presence of different concentrations of transition metal ions showed enhancement in fluorescence intensity of levosulpiride. The photophysical changes owing to the direct interaction between metal ion and the amide nitrogen of levosulpiride has been described in terms of CHEF (chelating enhancement fluorescence) effect. The absorption spectra of the drug at different pH exhibited two isosbestic points at 255 and 275 nm respectively, indicating the presence of three chemical species in solution. The ratio of the drug to metal ions is found to be 2: 1 and the log K of the resulting complex was determined spectrophotometrically and potentiometrically. The apparent ionization constant of levosulpiride is found to be 8.98. The low value of stability constant suggests that complexes may dissolve and the drug can be absorbed.