Abstract
Many operating parameters of ultrafiltration (UF) are playing a crucial role when using a polyethersulfone membrane to separate xylose reductase (XR) enzyme from reaction mixtures during xylitol synthesis. The present study focuses on the separation of XR enzyme using a cross-flow ultrafiltration (UF) membrane. The filtration process was analyzed using the three effective variables such as filtration time, cross-flow velocity (CFV), and the transmembrane pressure (TMP), which were ranging from 0 to 100 min, 0.52 to 1.2 cm/s and 1-1.6 bar, respectively. Then, using the resistance in series model, the hydraulic resistance for alkali chemical cleaning during XR separation was estimated. During separation, increased TMP showed a positive-flux effect as a driving force, however, fouling and polarized layer were more prominent under higher TMP. Increased CFV, on the other hand, was found more efficient in fouling control. In terms of the membrane cleaning techniques, an alkaline solution containing 0.1 M sodium hydroxide was shown to be the most effective substance in removing foulants from the membrane surface in this investigation. Cleaning with an alkaline solution resulted in a maximum flux recovery of 93% for xylose reductase separation. This work may serve as a useful guide to better understand the optimization parameters during XR separation and alleviating UF membrane fouling induced during XR separation.