Abstract
We report extracellular biosynthesis of silver nanoparticles by Fusarium solani (USM-3799), a phytopathogen causing disease in onion, when challenged with 1 mM silver nitrate (AgNO3). The formation of nanoparticles was characterized by visual observation followed by UV-Vis spectrophotometric analysis, which showed a peak at about 420 nm, which is very specific for silver nanoparticles. Further analysis carried out by Fourier Transform Infrared Spectroscopy (FTIR), provides evidence for the presence of proteins as capping agent, which helps in increasing the stability of the synthesized silver nanoparticles. Transmission Electron Microscopy (TEM) investigations confirmed that silver nanoparticles were formed. The synthesized silver nanoparticles were found to be polydispersed, spherical in the range of 5-35 nm with average diameter of 16.23 nm. Extracellular synthesis of nanoparticles could be highly advantageous from the point of view of synthesis in large quantities and easy downstream processing.