Abstract
Sickle Cell Anemia is one of those diseases for which cure or treatment for complete eradication is not available till date. The patients with the disease suffer prom a peculiar shape of the RBC. The complication mostly involved are, breathing difficulties, Joint pains, arthritis, bone infarctions and severe infections. In order to develop a screening test for the prognosis of Sickle Cell Anemia, the molecular basis of the disease is studied. The objective of the work was to detect the Single Nucleotide Polymorphism in the beta hemoglobin gene corresponding to the rs334 A to T/G/C in db SNP. Samples were processed to extract the DNA which was further purified and subjected for Amplification using specific primers developed towards the Hemoglobin beta gene that can amplify the region including the SNP site. Based on the results of the sequencing the SNPs were identified and can thus be used in the future prognosis of the condition. The work can further be extended with more samples and extensive data analysis.