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Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2
Journal article   Open access  Peer reviewed

Generation of four H1 hESC sublines carrying a hemizygous knock-out/mutant MECP2

Ruizhu Zeng, Harwin Sidik, Kim S Robinson, Franklin L Zhong, Bruno Reversade and Mahmoud A Pouladi
Stem cell research, Vol.40, pp.101533-101533
01/10/2019
DOI: https://doi.org/10.1016/j.scr.2019.101533
PMID: 31450191

Abstract

Cell Differentiation Cell Line CRISPR-Cas Systems - genetics Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Exons Gene Editing Humans Karyotype Methyl-CpG-Binding Protein 2 - genetics Rett Syndrome - genetics Rett Syndrome - pathology
Rett syndrome (RTT) is a childhood neurodevelopmental disorder caused by mutations in MECP2. To study the molecular mechanisms underlying RTT, four sublines of H1 hESCs were generated, carrying a hemizygous knockout or mutant allele of MECP2. Exons 3 and 4 of MECP2 were targeted using the CRISPR/Cas9 nuclease system.
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https://doi.org/10.1016/j.scr.2019.101533View
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