Abstract
Background: Chronic kidney disease is a common medical problem in Saudi Arabia. This study aimed to screen for HFE gene mutation as a possible risk factor for end-stage CKD. Methodology: Peripheral blood was collected in 4.5 mL ETA tube from 5 patients (all men, mean age 50 years, range 46-62 years) who attended the University of Ha'il's Medical caravan for Kidney failure chair. Applying Histopaque (1.083, Sigma, USA) leukocytes were isolated following the manufacturer's instructions. Results: The melting temperature depends upon length as well as the G+C of the PCR product contents. The wild type respective Tm is 55.80 degrees C while S65C is 50.70 degrees C and H63D 64.87 degrees C +/- 2.5 degrees C. The melting temperature (Mt) is presented by the two samples of the 5 examined were heterozygous for the mutation H63D (patients 3 (yellow) color and patient 2 (green). Conclusion: The findings of the current study suggesting the role of the hereditary hemochromatosis gene in the etiology of Chronic Kidney Failure in the Saudi Population. Further genetic studies with elevated sample size deemed important to confirm the role of the HFE gene mutation in the etiology of CKD, which mostly terminate in chronic renal failure.