Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

Herman Pel; Johannes de Winde; David Archer; Paul Dyer; Gerald Hofmann; Peter Schaap; Geoffrey Turner; Ronald de Vries; Richard Albang; Kaj Albermann; Mikael Andersen; Jannick Bendtsen; Jacques Benen; Marco van den Berg; Stefaan Breestraat; Mark Caddick; Roland Contreras; Michael Cornell; Pedro Coutinho; Etienne Danchin; Alfons Debets; Peter Dekker; Piet van Dijck; Alard van Dijk; Lubbert Dijkhuizen; Arnold Driessen; Christophe D'Enfert; Steven Geysens; Coenie Goosen; Gert Groot; Piet de Groot; Thomas Guillemette; Bernard Henrissat; Marga Herweijer; Johannes van den Hombergh; Cees van den Hondel; Rene van Der Heijden; Rachel van Der Kaaij; Frans Klis; Harrie Kools; Christian Kubicek; Patricia van Kuyk; Jürgen Lauber; Xin Lu; Marc van den Maarel; Rogier Meulenberg; Hildegard Menke; Martin Mortimer; Jens Nielsen; Stephen Oliver; Maurien Olsthoorn; Karoly Pal; Noël van Peij; Arthur Ram; Ursula Rinas; Johannes Roubos; Cees Sagt; Monika Schmoll; Jibin Sun; David Ussery; János Varga; Wouter Vervecken; Peter van de Vondervoort; Holger Wedler; Han Wösten; An-Ping Zeng; Albert van Ooyen; Jaap Visser; Hein Stam

doi:10.1038/nbt1282

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Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

Journal article

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Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88

Herman Pel, Johannes de Winde, David Archer, Paul Dyer, Gerald Hofmann, Peter Schaap, Geoffrey Turner, Ronald de Vries, Richard Albang, Kaj Albermann, …

Nature biotechnology, Vol.25(2), pp.221-231

01/02/2007

DOI: https://doi.org/10.1038/nbt1282

PMID: 17259976

Abstract

Biotechnology

Life Sciences

The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.

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Title: Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
Creators - without role: Herman Pel - DSM
Johannes de Winde - DSM
David Archer - University of Nottingham
Paul Dyer - University of Nottingham
Gerald Hofmann - Technical University of Denmark
Peter Schaap - Wageningen University & Research
Geoffrey Turner - University of Sheffield
Ronald de Vries - Utrecht University
Richard Albang - Biomax Informatics
Kaj Albermann - Biomax Informatics
Mikael Andersen - Technical University of Denmark
Jannick Bendtsen - CLC
Jacques Benen - Wageningen University & Research
Marco van den Berg - DSM
Stefaan Breestraat - DSM
Mark Caddick - University of Liverpool
Roland Contreras - Ghent University
Michael Cornell - University of Manchester
Pedro Coutinho - Architecture et Fonction des Macromolécules Biologiques
Etienne Danchin - Architecture et Fonction des Macromolécules Biologiques
Alfons Debets - Laboratory of Genetics
Peter Dekker - DSM
Piet van Dijck - DSM
Alard van Dijk - DSM
Lubbert Dijkhuizen - University of Groningen
Arnold Driessen - University of Groningen
Christophe D'Enfert - Institut Pasteur
Steven Geysens - Ghent University
Coenie Goosen - University of Groningen
Gert Groot - DSM
Piet de Groot - Vrije Universiteit Amsterdam
Thomas Guillemette - Université d'Angers
Bernard Henrissat - Architecture et Fonction des Macromolécules Biologiques
Marga Herweijer - DSM
Johannes van den Hombergh - DSM
Cees van den Hondel - Leiden University
Rene van Der Heijden - Radboud University Medical Center [Nijmegen]
Rachel van Der Kaaij - University of Groningen
Frans Klis - University of Amsterdam
Harrie Kools - Wageningen University & Research
Christian Kubicek - TU Wien
Patricia van Kuyk - Leiden University
Jürgen Lauber - Qiagen
Xin Lu - Helmholtz Centre for Infection Research
Marc van den Maarel - Centre for Carbohydrate Bioprocessing
Rogier Meulenberg - DSM
Hildegard Menke - DSM
Martin Mortimer - University of Liverpool
Jens Nielsen - Technical University of Denmark
Stephen Oliver - University of Manchester
Maurien Olsthoorn - DSM
Karoly Pal - University of Szeged
Noël van Peij - DSM
Arthur Ram - Leiden University
Ursula Rinas - Helmholtz Centre for Infection Research
Johannes Roubos - DSM
Cees Sagt - DSM
Monika Schmoll - TU Wien
Jibin Sun - Helmholtz Centre for Infection Research
David Ussery - Technical University of Denmark
János Varga - University of Szeged
Wouter Vervecken - Ghent University
Peter van de Vondervoort - Leiden University
Holger Wedler - Qiagen
Han Wösten - Utrecht University
An-Ping Zeng - Helmholtz Centre for Infection Research
Albert van Ooyen - DSM
Jaap Visser - Fungal Genetics and Technology Consultancy
Hein Stam - DSM
Publication Details: Nature biotechnology, Vol.25(2), pp.221-231
Publisher: Nature Publishing Group
Identifiers: 9936871608331
Academic Unit: King Abdulaziz University
Language: English
Resource Type: Journal article