Abstract
Background Low-risk HPV infection has not been the subject of epigenetic investigation. The present study was carried out in order to investigate the methylation status of CpG sites in non-genital cutaneous warts. Methods Genomic DNA was extracted from 24 paired epidermal samples of warts and normal skin. DNA samples were bisulfite converted and underwent genome-wide methylation profiling using the Infinium MethylationEPIC BeadChip Kit. Results From a total of 844,234 CpG sites, 56,960 and 43,040 CpG sites were found to be hypo- and hypermethylated, respectively, in non-genital cutaneous warts. The most differentially methylated CpG sites in warts were located within theC10orf26,FAM83H-AS1,ZNF644,LINC00702,GSAP,STAT5A,HDAC4,NCALD, andEXOC4genes. Conclusion Non-genital cutaneous warts exhibit a unique CpG methylation signature.