Abstract
The use and manufacturing of silver nanoparticles (AgNPs) have recently received considerable attention in many fields. Methicillin resistant Staphylococcus aureus (MRSA) causes infectious diseases in humans. Therefore, the aim of this work was to bio-synthesize AgNPs using the
cyanobacteria Phormidium sp. extract and to use this extract with AgNPs to kill MRSA. Extracellular bio-formation of AgNPs by Phormidium sp. was scrutinized at various pH points and temperatures. Then, characterization and assessment of the antimicrobial power of the produced
particles were performed. Extract and extract + AgNPs were screened using Fourier Transform Infra-Red (FT-IR) spectroscopy and electron microscopy. The manufacturing and fastness of AgNPs were monitored visually (by color change) and spectroscopically. FT-IR analysis uncovered the biomolecules
in the extract that caused the reduction of silver ions, protected them from aggregation and supplied them with high stability. Results indicated that AgNPs are spheres of 9 nm diameter. AgNPs combined with the extract proved to be a powerful antibacterial factor against MRSA. Consequently,
biosynthesis of silver nano-spheres using Phormidium sp. with high antagonistic potency against MRSA is considered as an eco-friendly, time-saving strategy and with potential medicinal significance.