Abstract
In this study, an eco-friendly and low-cost procedure for the in situ fabrication of Cu nanoparticles by using chitosan/alginate hydrogel. The prepared Cu NPs@CS/Alg nanocomposite were characterized by advanced physicochemical techniques like Fourier Transformed Infrared spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy (EDX) and X-ray Diffraction (XRD) study. It has been established that chitosan/alginate-capped gold nanoparticles have a spherical shape with a mean diameter from 10 to 20 nm. In the cellular and molecular part of the recent study, the treated cells with Cu NPs@CS/Alg nanocomposite were assessed by MTT assay for 48 h about the cytotoxicity and anti-human breast cancer properties on normal (HUVEC) and breast cancer cell lines i.e. infiltrating lobular carcinoma of breast (UACC-3133), inflammatory carcinoma of the breast (UACC-732), and metastatic carcinoma (MDA-MB-453). In the antioxidant test, the IC50 of Cu NPs@CS/Alg nanocomposite and BHT against DPPH free radicals were 344 and 193 mu g/mL, respectively. The IC50 of Cu NPs@CS/Alg nanocomposite were 297, 386, and 359 mu g/ mL against KYSE-270, OE33, and ESO26 cell lines, respectively. The viability of malignant breast cell line reduced dose-dependently in the presence of Cu NPs@CS/Alg nanocomposite. (C) 2021 Published by Elsevier B.V. on behalf of King Saud University.