Abstract
A natural, accurate, extremely rapid, and precise spectrofluorometric method has been developed and validated for determination of salmeterol (SAL) xinafoate in its medicinal commercial form and spiked human plasma. The native SAL fluorescence has been measured at 415 nm (after 340 nm as excitation) in distilled water. Different factors affecting the native fluorescence consistency of SAL were surveyed and optimized. The suggested procedure was capable of SAL determination over concentrations ranging 200–2000 ng.mL−1 with excellent correlation coefficient of 0.9995. The limits of detection and quantification were estimated as 44.44 ng mL−1 and 134.66 ng mL−1, respectively. The method has good accuracy and precision. The green spectrofluorometric method was used for determination of SAL in its commercial preparations and the results were in accordance with other reported methods regarding accuracy and precision. Moreover, the proposed procedure was enforced for stability indicating assay of SAL and for SAL determination in spiked human plasma. Nearly no cost, high sensitivity, and wide application make the proposed method ideally suited for analysis of SAL in quality control laboratories.
The fluorescence spectra of Salmeterol (1000 µg mL−1); In which: (A, B) are excitation and emission spectra of blank respectively. (C, D) are excitation and emission of cited drug respectively.