Abstract
The aim of this work was to develop a new biological process for the fabrication of silver nanoparticles (Ag-NPs) using the extract of a xerophytic plant (Neurada procumbens) as a reducing agent. The fabricated Ag-NPs can effectively control multi-drug resistant gram-negative rods (MDR-GNRs), which were isolated from the Buraidah Central Hospital (BCH), Saudi Arabia. The predominant MDR-GNR bacteria included Klebsiella pneumoniae, Acinetobacter baumannii, and Escherichia coli. The Ag-NPs were examined by diverse spectral and analytical techniques, and then their antibacterial effects against MDR bacteria were determined. The UV spectroscopy showed absorption peaks between 400 and 455 nm specific to AgNPs. The SEM and TEM results showed a smallsize of Ag-NPs in a range of about 20-50 nm. The XRD pattern showed three major distinctive peaks of Ag-NPs at 38.1 degrees, 44.3 degrees and 64.4 degrees confirming the presence of Ag-NPs. Three multidrug-resistant bacterial strains were used to determine antimicrobial activity by the well-diffusion method. The present workillusrates that synthesized Ag-NPs from the aqueous leaf extract of Neurada procumbens show considerable antibacterial potential against MDR clinical isolates from different patient samples at BCH. (C) 2019 Published by Elsevier B.V. on behalf of King Saud University.