Abstract
Antimicrobial resistance is a worldwide problem. Pathogenic microorganisms develop antibiotic resistance as a result of exposure to inappropriate quantity of antibiotics and accumulation of mutations. Bio-friendly nanomaterial scan be developed as antimicrobial agents. Present study aimed to investigate the activity of biosynthesized silver nanoparticles (AgNPs) obtained from carrot extract of three different regions namely Gilgit, Haripur and Sheikhupura from Pakistan, having significant difference in climate and altitude. UV–visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis were carried out for the morphological characterization of synthesized nanoparticles. Analysis of XRD revealed crystalline nature of AgNPs and average size calculated was about 22, 17 and 10 nm from Gilgit, Haripur and Sheikhupura samples respectively. The antibacterial efficacy of AgNPs was carried out against 7 American type culture collection strains of pathogenic bacteria and three macrolide resistant clinical isolates with promising results. Moreover, antioxidant activity indicated maximum DPPH radical scavenging effects as 47 % using a concentration of 250 μg/mL. Hemolysis assays indicated biocompatibility of AgNPs at lower concentration like 7 μg/mL and 15 μg/mL. Comparative analysis of bioactivity from different sites of sampling had indicated minute differences which may be due to the change in altitude, soil texture and other local environmental changes.