Abstract
A highly sensitive and simple spectrofluorimetric method has been developed and validated for the determination of stiripentol (STP) in its pharmaceutical formulations and human urine. The proposed method is based on the investigation of the fluorescence spectral behaviour of STP in methanol using synchronous scan technique (Delta lambda = 80 nm, 343 nm). The fluorescence-concentration plot was rectilinear over the range 10-70 ng/mL, with lower detection limit of 2ng/mL. The proposed method was successfully applied to the assay of commercial capsules, spiked urine samples as well as weight variation testing. The application of the proposed method was extended to test the in-vitro drug release of STP capsules, according to USP guidelines.