Abstract
Favipiravir (FVP) structurally is an analog of pyrazine and showed its antiviral actions against a diverse species of viruses. Due to this fact, it was chosen as a potential candidate to be further investigated to cure COVID-19. A simple, accurate, rapid, precise, high-performance liquid chromatography (HPLC) method has been established for quality control assurance of FVP in pharmaceutical preparations. Column no C8 (150 mm x 4.6 mm; 5 mu m) was used for such separation analysis. A combination of acetonitrile and phosphate buffer was used in 10:90 ratios as the mobile phase (90:10, v/v) having a flow rate of 1 mL / min. further, it was detected at 220 nm, and the temperature was maintained at 25 degrees C. The run time was then set at 10 min. A linear relationship of R-2 0.998 was confirmed while drawing a graph between FVP concentration and peak area that has a concentration range of 40-200 ppm. The developed method is sensitive (limits of detection and quantification are 1.83 ppm and 5.75 ppm respectively). Three injections at concentrations of 80,100 and 120 (ppm) were injected the same day to ensure the precision of the method. The prepared solution remains stable for 24 h. The suggested method has been successfully useful for the quantification of FVP in pharmaceutical formulations.