Abstract
A reversed phase high-performance liquid chromatographic method for the analysis of meclofenamate sodium in plasma was developed. Diclofenac was used as the internal standard. The chromatography was performed using a resolve C
18
column; the mobile phase consisted of 60:40% methanol to water and adjusted to pH 3.0 using acetic acid; a flow rate of 1.5 ml/min; and UV detection at 270 nm. Retention times were 3.6 and 5.9 min for diclofenac and meclofenamate, respectively. The mean (±SD) absolute and relative recovery of meclofenamate were found to be 96.49±0.59 and 100.48±0.73, respectively. The minimum detectable concentration of meclofenamate by this method was 150 ng/ml sample. The sensitivity obtained should enable the use of the method in future bioequivalency and/or pharmacokinetic studies.